Esta página foi editada pela última vez às 12h00min de 3 de dezembro de 2013. Data from A, B, and C are representative of three experiments, and from D of two experiments. Article The effect of sensitization and challenge with 10 μg of BtE per injection on lung histology is seen in Figures 5F and 5G. Polo día, o home perde 1 g destes pedazos de pel. Storage mites are a possible cause of allergic disorders and mostly present among agriculture population. All authors read and approved the final manuscript. Blomia tropicalis é uma espécie de ácaro presente em amostras de poeira intradomiciliar, comumente encontrado em regiões de clima tropical, como o Brasil, infestando residências. (A) Mucus Index in the bronchi. Sabit M, Ramos JDA, Alejandro GJ, and Gala C. Seasonal distribution of airborne pollen in Manila, Philippines, and the effect of meteorological factors to its daily concentrations.Aerobiologia 2015 (DOI 10.1007/s10453-015-9414-2). Ann Allergy Asthma Immunol 2003,90(6):664–8. (A) Total IgE. VMGS, TM and PRSO helped in the experiments on different mouse strains. © 2021 BioMed Central Ltd unless otherwise stated. Entry date: September 24, 2002 12:00 +1GMT: Last update: July 24, 2019 20:03 +1GMT: Allergome Code: 152 On the other hand, OVA sensitization led to higher mucus production, and serum levels of specific IgE, IgG1 and IgG2a than BtE sensitization. *p < 0.05, **p < 0.01, and ***p < 0.001 for the indicated tested groups (Tukey's test). Total cell counts were carried out using a Neubauer chamber. Fernvik E, Peltre G, Sénéchal H, Vargaftig BB: Effects of birch pollen and traffic particulate matter on Th2 cytokines, immunoglobulin E levels and bronchial hyper-responsiveness in mice. Respir Res 11, 51 (2010). Reitor Miguel Calmon, Sem n°. The first objective of the present work was to study the murine allergic response to B. tropicalis using a short-term immunization protocol. (D) Anti-B. (C) Level of eosinophil peroxidase (EPO) activity in BALF. Among the mites, Dermatophagoides pteronyssinus and Blomia tropicalis are the main sources of allergens in sub-tropical and tropical regions of the world [4–6]. Allergens derived from this mite cause allergic reactions, including asthma and atopy. Canela, Salvador, Bahia, CEP 40110902, Brasil, Tiana Baqueiro, Virgínia MG Silva, Ana T Cerqueira-Lima, Camila A Figueiredo & Neuza M Alcântara-Neves, Núcleo de Tecnologia em Saúde, Instituto Multidisciplinar em Saúde, Universidade Federal da Bahia, Av. Mice of all strains had lung inflammatory-cell infiltration and increased levels of anti-BtE IgE antibodies, but these responses were significantly more intense in A/J mice than in CBA/J, BALB/c or C57BL/6J mice. International Archives of Allergy and Immunology 2009, 149:25-32. Chapman MD, Smith AM, Vailes LD, Arruda LK: Defined epitopes: in vivo and in vitro studies using recombinant allergens. This model may constitute a better approximation to a natural allergenic sensitization, in which allergic individuals tend to be exposed to low allergen doses, independently of the entry route, than the so far published experimental murine models, that use higher BtE doses [[14–16] and ]. Following centrifugation (400 g, 5 min, 4°C), supernatants of the BALF were collected and stored at -70°C for subsequent measurement of cytokine content and the pellets were used for the measurement of eosinophil peroxidase (EPO) activity. Clin Exp Allergy 2002, 32:602–611. Int Arch Allergy Immunol 1998,115(2):144–9. Springer Nature. P > 0.05 is not represented. Terms and Conditions, An aliquot of the BALF cells was washed three times by centrifugation, and the cell pellet resuspended in PBS-BSA. Clin Exp Allergy 2002,32(11):1667–74. Google Scholar. Thus, sensitization to B. tropicalis allergens is considered an independent and important cause of allergy [4, 8]. Blomia tropicalis van Bronswijk, de Cock & Oshim This mite is common in warmer regions and is mainly regarded as a house dust mites that can cause allergies. Our mission is to enable our customers to make the world healthier, cleaner, and safer. Therefore, the journal welcomes submissions from all over the world. Although a mouse model for B. tropicalis extract (Bt E)-induced asthma has been described, no study comparing different mouse strains in this asthma model has been reported. Castro Almarales RL, Mateo Morejon M, Naranjo Robalino RM, Navarro Viltre BI, Alvarez Castello M, Ronquillo Diaz M, Garcia Gomez I, Oliva Diaz Y, Gonzalez Leon M, Rodriguez Canosa JS, Labrada Rosado A: Correlation between skin tests to Dermatophagoides pteronyssinus, Dermatophagoides siboney and Blomia tropicalis in Cuban asthmatics. In A and C, each symbol corresponds to the result obtained from an individual animal. Google Scholar. Cookies policy. PubMed Google Scholar. In our model, eosinophils were the preponderant cells in the airways, however similar numbers of neutrophils and eosinophils were found in airways when the animals were sensitized without alum (data not shown). (E) IL-4 concentration in BALF. TB conducted the majority of the experiments involving different mouse strains, the OVA × BtE comparison experiments and wrote the first manuscript draft. After the observation that A/J was the best mouse strain for BtE-induced asthma, we immunized these mice with a low-dose (10 μg per injection) of BtE instead of the 100 μg dose per injection used in the previous experiments. Espesye sa uhong sakop sa division nga Ascomycota ang Candida tropicalis.Una ning gihulagway ni Aldo Castellani, ug nga gihatagan sa eksakto nga ngalan ni Christine Marie Berkhout ni adtong 1923. The relevance and reproducibility of experimental animal models of allergy depends on the genetic background of the animal, the molecular composition of the allergen and the experimental protocol. Animal models that mimic the immunological and pulmonary inflammation features observed in human asthma are important tools to dissect the basic cellular and molecular mechanisms involved in the initiation and control of allergy . IFN-γ and IL-10 concentrations in the BALF from BtE-sensitized or saline-treated mice of all tested mouse strains were low, and no statistically significant differences were found among the studied groups and their negative controls (data not shown). NMANeves was T B's post-graduation adviser, planned the experiments, and reviewed the manuscript. (D) Bt-sensitized group. EG and RB helped in the experiments with OVA- and BtE-induced asthma models. The production of IL-4 in BtE-sensitized and challenged mice was higher in A/J when compared with the other studied mouse strains (Tukey's test, p < 0.05; Figure 1E); it was followed by the production in CBA/J mice (p < 0.01, Tukey's test). Thus, it appears that alum is required to achieve fully polarized Th2 responses to BtE. 室塵 蟎 （ ㄇㄢˊ ） 屬（学名： Dermatophagoides ）（英語： house dust mite ）是一種8隻腳的微小動物，屬於節肢動物門 蛛形綱 恙蟎目 塵蟎科。 是台灣兒童主要的過敏原之一 。. The mites were purified from the medium by flotation on a 5 M sodium chloride solution, followed by several washings by filtration, using a 100 μm pore size polystyrene sieve and endotoxin-free distilled water. IgE antibody serum levels were estimated by PCA reaction, as described by Mota and Wong . J Allergy Clin Immunol 1997,100(6 Pt 1):S2–24. Ácaro é a designação comum dada aos animais pertencentes à subclasse Acari da classe Arachnida (os aracnídeos).A palavra acari deriva do grego akares, 'pequeno'.A maioria dos adultos mede entre 0,25 e 0,75 mm de comprimento, embora existam espécies ainda menores. Jumlah Blomia tropicalis cukup besar ditemukan pada rumah-rumah di daerah tropis dan subtropis (Arlian et al., 1993). The rats were painlessly killed 30 min later, and the reciprocal of the highest serum dilution to produce a blue spot with more than 5 mm of diameter was considered the PCA titer. Int Arch Allergy Immunol 1997,113(1–3):102–4. Tissues sections of 5-μm were then stained with periodic acid-Schiff (PAS) for the evaluation of mucus production. The data are representative of three independent experiments. It is an in vitro quantitative assay that is intended to be used in conjunction with other clinical information to aid in the diagnosis of allergic diseases. On the other hand, a protocol developed by Eum and collaborators , using OVA, showed that shortening the duration of the allergic protocol did not affect the immunopathological features of the experimental disease, when it was compared with classical protocols . This article is published under license to BioMed Central Ltd. (C) Level of eosinophil peroxidase (EPO) activity in BALF. Neuza M Alcântara-Neves. http://creativecommons.org/licenses/by/2.0. Lowry OH, Rosenbrough NJ, Faar AL, Randall RJ: Protein measurement with the folin phenol reagent. Although a mouse model for B. tropicalis extract (BtE)-induced asthma has been described, no study comparing different mouse strains in this asthma model has been reported. Fernandes-Caldas E, Puerta L, Mercado D, Lockey RF: Mite fauna, Der p I, Der f I and Blomia tropicalis allergen levels in a tropical environment. The following parameters were used to measure the immune response in mice of four inbred strains (CBA/J, BALB/c, A/J and C57Bl/6): (i) the total number of leukocytes and eosinophils in the bronchoalveolar lavage fluid (BALF); (ii) the concentration of IL-4 and IL-13 cytokines and eosinophil peroxidase (EPO) in the BALF; (iii) the serum levels of anti-B. The tracheas of the dead mice were cannulated and the BALF collected in 0.5 mL of PBS containing 1% of bovine serum albumin (Sigma Chemical Co., St. Louis, MO, USA; PBS-BSA). In order to verify differences among more than two mouse groups, the results were analyzed using the one-way ANOVA test and the Tukey's post test. This study included the comparison of the allergic responses elicited by BtE with those elicited by ovalbumin in mice of the strain that responded better to BtE sensitization. Google Scholar. Sensitized A/Sn mice fed with Bt or Dp showed a significant … Sanchez-Borges M, Capriles-Hulett A, Caballero-Fonseca F, Fernandez-Caldas E: Mite and cockroach sensitization in allergic patients from Caracas, Venezuela. Eur J Immunol 2007, 37:1170–1173. Geographical distribution See common geographical background to mites in our Scientific Documents (link to the right). de Guia RM, Echavez MDJ, Gaw ELC, Gomez MRR, Lopez KAJ, Mendoza RCM , Rapsing JMC, Retreta DP, Tubog CMB, Ventolero MH, Yao CL, Ramos JDA. Clin Exp Allergy 1993,23(4):292–7. Detectable dust mite allergen was found in the beds of about 84% of surveyed United States homes. Lipids from the lysate were extracted and discarded by five or six ether extractions. PBS containing 1% of bovine serum albumin. Although the total cell counts in BALF were higher in sensitized A/J mice than in the other sensitized mouse strains, the differences were not statistically significant (p > 0.05, ANOVA test; Figure 1A). Prof. Lineu Prestes, 1730, Cidade Universitária, Butantã, CEP 05508-900, Brazil, Momtchilo Russo, Eliane Gomes & Renato Barboza, Centro de Pesquisas Gonçalo Moniz, Fundação Oswaldo Cruz, Rua Waldemar Falcão, 121, Brotas, Salvador, Bahia, CEP 40296710, Brazil, Virgínia MG Silva, Thayna Meirelles, Pablo RS Oliveira & Lain Pontes-de-Carvalho, You can also search for this author in PubMed Tsai JJ, Wu HH, Shen HD, Hsu EL, Wang SR: Sensitization to Blomia tropicalis among asthmatic patients in Taiwan. Sato MN, Oliveira CR, Futata EA, Victor JR, Maciel M, Fusaro AE, Carvalho AF, Duarte AJ: Oral tolerance induction to Dermatophagoides pteronyssinus and Blomia tropicalis in sensitized mice: occurrence of natural autoantibodies to immunoglobulin E. (A) Total cell count in the BALF. No differences in numbers of macrophage, lymphocyte and neutrophils in the BALF were observed among the mice of all four strains (p > 0.05, ANOVA; data not shown). Keller AC, Mucida D, Gomes E, Faquim-Mauro E, Faria AMC, Rodriguez D, Russo M: Hierarchial suppression of asthma-like responses by mucosal tolerance. Immunization of A/J mice with BtE induced a more intense airway eosinophil influx, higher levels of total IgE, similar airway hyperreactivity to methacholine but less intense mucous production, and lower levels of specific IgE, IgG1 and IgG2 antibodies than sensitization with OVA.
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